Synergistic Effect of Theophylline and Procaterol On Interleukin-5-Induced Degranulation from Human Eosinophils

Inhibiting the release of toxic granule proteins from eosinophils is a possible means of treating allergic inflammation. This study was performed to examine whether procaterol and theophylline, commonly used bronchodilators in asthma, inhibit eosinophil degranulation induced by interleukin (IL)-5. P...

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Bibliographic Details
Published in:The Journal of asthma 2002-01, Vol.39 (1), p.21-27
Main Authors: Fujisawa, Takao, Kato, Yoshiko, Terada, Akihiko, Iguchi, Kosei, Kamiya, Hitoshi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bronchodilator Agents - pharmacology
Cell Degranulation - drug effects
Degranulation
Dexamethasone - pharmacology
Drug Synergism
Eosinophil
Eosinophils - physiology
Humans
Interleukin-5
Interleukin-5 - pharmacology
Medical sciences
Pharmacology. Drug treatments
Procaterol
Procaterol - pharmacology
Respiratory system
Theophylline
Theophylline - pharmacology
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Summary:Inhibiting the release of toxic granule proteins from eosinophils is a possible means of treating allergic inflammation. This study was performed to examine whether procaterol and theophylline, commonly used bronchodilators in asthma, inhibit eosinophil degranulation induced by interleukin (IL)-5. Purified eosinophils from patients with asthma were incubated with IL-5 for 24 hr in the presence of theophylline, procaterol, combinations of theophylline and procaterol, or dexamethasone. Levels of eosinophil-derived neurotoxin (EDN) in the supernatants were measured with radioimmunoassay. Theophylline inhibited IL-5-induced release of EDN in a concentration-dependent manner. Procaterol inhibited degranulation only at high concentrations. However, procaterol at 10−9M and 10−8 M, which are physiologic concentrations, together with theophylline at 10−5 M, which is a concentration commonly found in the serum of patients receiving low-dose theophylline, inhibited degranulation by 43.8%. This finding indicates that theophylline and procaterol have synergistic effects. The inhibition was comparable to that with dexamethasone at 10−9 M. Our results suggest that a combination of low-dose theophylline and procaterol exhibits antiinflammatory effects in asthma by inhibiting eosinophil-effector functions.
ISSN:0277-0903
1532-4303
DOI:10.1081/JAS-120000803