Type I hair cell regeneration induced by Math1 gene transfer following neomycin ototoxicity in rat vestibular sensory epithelium

Abstract Conclusion: In the current study, hair cells of vestibular terminal organs in rats were completely eliminated with trans-scala vestibuli injection of neomycin, and then the Math1 gene was transferred. It was shown that type I vestibular hair cells were regenerated and synapses were formed....

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Published in:Acta oto-laryngologica 2012-08, Vol.132 (8), p.819-828
Main Authors: Xu, Jin-Cao, Huang, De-liang, Hou, Zhao-Hui, Guo, Wei-Wei, Sun, Jian-He, Zhao, Li-Dong, Yu, Ning, Young, Wie-Yen, He, David Zhi-Zhou, Yang, Shi-Ming
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Basic Helix-Loop-Helix Transcription Factors - biosynthesis
Basic Helix-Loop-Helix Transcription Factors - genetics
Biological and medical sciences
Disease Models, Animal
Drug toxicity and drugs side effects treatment
Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology
Female
gene therapy
Gene Transfer Techniques
Genetic Therapy - methods
Hair Cells, Vestibular - physiology
Hair Cells, Vestibular - ultrastructure
Hearing loss
Hearing Loss, Sensorineural - chemically induced
Hearing Loss, Sensorineural - pathology
Hearing Loss, Sensorineural - therapy
Immunohistochemistry
Medical sciences
Microscopy, Confocal
Microscopy, Electron, Scanning Transmission
Mitosis
Neomycin - toxicity
Non tumoral diseases
Otorhinolaryngology. Stomatology
Pharmacology. Drug treatments
Rats
Rats, Wistar
Regeneration - physiology
synaptic linkage
Toxicity: respiratory system, ent, stomatology
vestibular function
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Summary:Abstract Conclusion: In the current study, hair cells of vestibular terminal organs in rats were completely eliminated with trans-scala vestibuli injection of neomycin, and then the Math1 gene was transferred. It was shown that type I vestibular hair cells were regenerated and synapses were formed. Objectives: The objective of this study was to identify the cell type of the regenerated vestibular hair cells and relative innervation and synaptic linkage after hair cells of vestibular terminal organs in rats were completely eliminated. Methods: Neomycin injection was used to eliminate all the vestibular terminal organs, and then the animals were treated with an injection of Ad-Math1-EGFP in the scala vestibuli of the cochlea. Results: Math1 gene transfer into the inner ear induced type I hair cell regeneration and synaptic formation. However, neither the number nor the appearance of the hair cells was normal.
ISSN:0001-6489
1651-2251
DOI:10.3109/00016489.2012.673233