Ultrastructural Characteristics of Central Neurocytoma in Cell Culture

The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-...

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Bibliographic Details
Published in:Ultrastructural pathology 1997, Vol.21 (4), p.393-404
Main Authors: Lee, Min-Cheol, Nam, Jong-Hee, Choi, Chan, Park, Chang-Soo, Juhng, Sang-Woo, Yang, Ki-Hwa, Suh, Chae-Hong, Kim, Seung U., Yang, Bok-Soog
Format: Article
Language:English
Subjects:
Adolescent
Adult
Brain Neoplasms - therapy
Brain Neoplasms - ultrastructure
cell culture
central neurocytoma
Cerebral Ventricles - ultrastructure
electron microscopy
Female
Humans
Immunohistochemistry
Magnetic Resonance Imaging
Male
Microscopy, Electron
Neurocytoma - therapy
Neurocytoma - ultrastructure
Tumor Cells, Cultured
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Summary:The study was performed to determine the ultrastructural characteristics of central neurocytoma and its features in primary cell culture. Fresh tissue from three tumors was mechanically and enzymatically dissociated into individual cells, which were cultured onto poly-L-lysine precoated Aclar cover-slips in the media. The tumor cells attached to the surface of the coverslips within 12 to 24 h and delicate cytoplasmic processes developed within 2 to 3 days. Electron microscopic examination of the cultured tumor cells and the tumor tissue supported neuronal origin. Combined tissue culture and electron microscopic study provides a rapid, reliable, and reproducible means for the diagnosis of central neurocytoma.
ISSN:0191-3123
1521-0758
DOI:10.3109/01913129709021937