Detection of Tubulointerstitial Nephritis Antigen (TIN-ag) in Lewis Rat

Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue...

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Bibliographic Details
Published in:Connective tissue research 1997, Vol.36 (3), p.223-229
Main Authors: Nelson, Todd R., Butkowski, Ralph J., Michael, Alfred F., Charonis, Aristidis S.
Format: Article
Language:English
Subjects:
Animals
autoimmunity
Blotting, Southern
Blotting, Western
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - isolation & purification
Cloning, Molecular
DNA, Complementary - isolation & purification
Lewis rat
Membrane Glycoproteins - genetics
Membrane Glycoproteins - isolation & purification
Nephritis, Interstitial - immunology
Northern blotting
Polymerase Chain Reaction
Protein Biosynthesis
Rabbits
Rats
Rats, Inbred Lew
Rats, Sprague-Dawley
RNA - isolation & purification
Southern blotting
Telomere-Binding Proteins
Transcription, Genetic
Tubulointerstitial nephritis antigen
Western blotting
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Summary:Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TIN-ag, with the site of most prominent expression the kidney tubular basement membrane. However, Lewis rat does not demonstrate any immunoreactivity for TIN-ag and does not develop tubuloinstersti-tial nephritis after injection of tubular basement membrane material. As TIN-ag would appear to be a molecule of biological significance, experiments were designed to explore the presence or absence of this macromolecule in the Lewis rat model. Southern blotting of Lewis rat genomic DNA revealed the presence of gene sequences corresponding to TIN-ag. RTPCR analysis of Lewis rat kidney cortex total RNA illustrated the expression of a TIN-ag gene product. Western blotting demonstrated the presence of TIN-ag protein forms in kidney cortical homogenates of Lewis rat. The data suggest either extensive epitope masking or expression polymorphism of TIN-ag in the Lewis rat.
ISSN:0300-8207
1607-8438
DOI:10.3109/03008209709160222