Detection of Treponema pallidum in Aqueous Humor by Real-time Polymerase Chain Reaction

Purpose: To report the additional clinical value of real-time Polymerase chain reaction (PCR) in the detection of Treponema pallidum in aqueous humor. Methods: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecu...

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Published in:Ocular immunology and inflammation 2011-04, Vol.19 (2), p.127-128
Main Authors: Cornut, Pierre-Loïc, Sobas, Chantal Roure, Perard, Laurent, De Bats, Flore, Salord, Hélène, Janin Manificat, Hélène, Denis, Philippe, Burillon, Carole
Format: Article
Language:English
Subjects:
aqueous humor
Aqueous Humor - microbiology
Computer Systems
Eye Infections, Bacterial - diagnosis
Humans
ocular syphilis
Panuveitis - microbiology
Polymerase Chain Reaction - methods
real-time PCR
Retinitis - microbiology
Syphilis - diagnosis
Treponema pallidum
Treponema pallidum - isolation & purification
uveitis
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Summary:Purpose: To report the additional clinical value of real-time Polymerase chain reaction (PCR) in the detection of Treponema pallidum in aqueous humor. Methods: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecutive patients with ocular syphilis. Results: Five patients presenting with optic neuritis (1), posterior placoid chorioretinitis (1), or panuveitis with retinitis (3) were tested. The PCR results were positive in the 3 cases of panuveitis and negative in the 2 other cases. Conclusion: RT-PCR for Treponema pallidum in aqueous humor is useful to confirm syphilitic panuveitis.
ISSN:0927-3948
1744-5078
DOI:10.3109/09273948.2010.531175