The progesterone and estrogen modify the uterine prolactin and prolactin receptor expression of hyperprolactinemic mice

• Published in: Gynecological endocrinology 2015-02, Vol.31 (2), p.148-151
• Main Authors: do Amaral, Vinícius Cestari, Carvalho, Kátia Candido, Maciel, Gustavo Arantes Rosa, Simoncini, Tommaso, da Silva, Priscilla Ludovico, Marcondes, Rodrigo Rodrigues, Soares, José Maria, Baracat, Edmund Chada
• Format: Article
• Language: English
• Subjects: Animals; Estradiol - blood; Estrogen; Estrogens - blood; Estrogens - pharmacology; Female; hyperprolactinemia; Hyperprolactinemia - blood; Hyperprolactinemia - metabolism; Hyperprolactinemia - pathology; Mice; Ovariectomy; progesterone; Progesterone - blood; Progesterone - pharmacology; Prolactin - blood; Prolactin - metabolism; prolactin receptor; Receptors, Prolactin - metabolism; Uterus - drug effects; Uterus - metabolism
• ISSN: 0951-3590; 1473-0766
• DOI: 10.3109/09513590.2014.967204

Abstract The aim of this study was to evaluate the effects of metoclopramide-induced hyperprolactinemia on the prolactin (PRL) and PRL receptor's expression in the uterus of mice. For this purpose, 49 Swiss mice were divided into the following groups: GrSS (non-ovariectomized mice given vehicle); GrMET (non-ovariectomized mice treated with metoclopramide); OvSS (ovariectomized mice given vehicle); OvMET (ovariectomized mice treated with metoclopramide); OvMET+17βE (ovariectomized mice treated with metoclopramide and 17β estradiol); OvMET+MP (ovariectomized mice treated with metoclopramide and micronized progesterone); OvMET+17βE+MP (ovariectomized mice treated with metoclopramide and a solution of 17β estradiol and micronized progesterone). Immunohistochemical analyzes were evaluated semi-quantitatively. Our results showed that GrMET, OvMET+MP, and OvMET+17βE+MP presented strong PRL expression. OvMET and OvMET+17βE presented mild reaction, while GrSS and OvSS presented weak reaction. Concerning PRL receptor, OvMET+MP and OvMET+17βE+MP showed strong reaction; GrMET, OvSS, and OvMET+17βE showed mild reaction; and GrSS and OvMET showed weak reaction. These findings suggest that progesterone alone or in combination with estrogen may increase the expression of uterine PRL and PRL receptor. Chinese abstract 本研究目的是评估胃复安所致高催乳素血症小鼠的子宫催乳素(PRL)和PRL受体表达的影响。49个瑞士小鼠分为以下组：GrSS(非去势小鼠赋形剂组)；GrMET（非去势小鼠胃复安治疗组)；OvSS(去势小鼠赋形剂组)；OvMET(去势小鼠胃复安治疗组)；OvMET+17βE (去势小鼠胃复安和17β雌二醇治疗组)；OvMET + MP(去势小鼠胃复安和微粒化孕酮治疗)； OvMET+17βE+MP（去势小鼠胃复安、17β雌二醇和微粒化孕酮溶液治疗组)。应用免疫组化法进行半定量测定。研究结果显示，GrMET， OvMET + MP，OvMET+17βE + MP显示PRL强阳性表达。OvMET和OvMET + +17βE显示中等表达,而GrSS和OvSS显示弱阳性表达。关于 PRL受体，OvMET + MP和OvMET + 17βE + MP显示强阳性表达；GrMET，OvSS和OvMET + 17βE 显示中等表达，而GrSS和OvMET显示弱阳性表达。这些研究表明，孕酮单独或结合雌激素可能增加子宫PRL和PRL受体的表达。