Microbial transformation of hydrocortisone by two fungal species Fusarium fujikuroi PTCC 5144 and Rhizomucor pusillus PTCC 5134

Abstract This paper examines the biotransformation of hydrocortisone (1) by Fusarium fujikuroi and Rhizomucor pusillus. These species have not previously been tested for hydrocortisone biotransformation. The metabolites produced during hydrocortisone biotransformation by these two fungi were 11β,17α...

Full description

Saved in:
Bibliographic Details
Published in:Biocatalysis and biotransformation 2014-06, Vol.32 (3), p.168-172
Main Authors: Ghasemi, Sabrieh, Kheyrabadi, Rasool, Habibi, Zohreh
Format: Article
Language:English
Subjects:
biotransformation
fungi
Fusarium fujikuroi
hydrocortisone
Rhizomucor pusillus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract This paper examines the biotransformation of hydrocortisone (1) by Fusarium fujikuroi and Rhizomucor pusillus. These species have not previously been tested for hydrocortisone biotransformation. The metabolites produced during hydrocortisone biotransformation by these two fungi were 11β,17α,20β,21-tetrahydroxypregn-4-en-3-one (2) and 11 β-hydroxyandrost-4-en-3,17-dione (3). Chemical structures were determined by spectroscopic methods. A time course study revealed that the disappearance of hydrocortisone was accompanied by the formation of metabolites 2 and 3. Metabolite 2 was produced as the major metabolite with high yield but the transformation to metabolite 3 was considerably lower, as determined by HPLC.
ISSN:1024-2422
1029-2446
DOI:10.3109/10242422.2014.913581