Role of the ERK signaling pathway in regulating vasopressin secretion in dehydrated rats

Abstract Dehydration activates the vasopressinergic system of the hypothalamus. We analyzed the effects of dehydration induced by water deprivation for 3 days on the activities of ERK1/2 and transcription factors, Elk1 and cAMP response element-binding protein (CREB) in vasopressinergic neurons, as...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2014-04, Vol.89 (3), p.199-208
Main Authors: Nikitina, LS, Dorofeeva, NA, Kirillova, OD, Korotkov, AA, Glazova, M, Chernigovskaya, EV
Format: Article
Language:English
Subjects:
Animals
CREB
Cyclic AMP Response Element-Binding Protein - metabolism
dehydration
Dehydration - metabolism
Gene Expression - physiology
hypothalamus
Hypothalamus - metabolism
Hypothalamus - pathology
Hypothalamus - secretion
kinesin
Male
MAP Kinase Signaling System - physiology
neurohypophysis
Rats, Wistar
Vasopressins - secretion
Water Deprivation - physiology
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Summary:Abstract Dehydration activates the vasopressinergic system of the hypothalamus. We analyzed the effects of dehydration induced by water deprivation for 3 days on the activities of ERK1/2 and transcription factors, Elk1 and cAMP response element-binding protein (CREB) in vasopressinergic neurons, as well as the distribution and level of the motor protein, kinesin, in the hypothalamo-neurohypophyseal system. We showed that dehydration resulted in enhanced vasopressin (VP) expression and activation of CREB, and increased the activity of the MEK/ERK/Elk1 pathway in magnocellular neurons of the supraoptic nucleus. The activation of VP secretion was associated also with accumulation of phospho-ERK1/2 in the VP-ergic terminals of the posterior lobe of the pituitary. Analysis of the amount and distribution of kinesin and SNAP25, the proteins associated with transport and secretion, demonstrated that dehydration enhanced kinesin content in the perikarya of magnocellular neurons in the supraoptic nucleus and decreased kinesin and SNAP25 levels in the posterior pituitary. ERK1/2 and ERK1/2-dependent transcription factors, Elk1 and CREB, participate in the regulation of dehydration-evoked VP expression. We propose that ERK1/2 and kinesin participate in regulation of anterograde transport of VP dense core vesicles.
ISSN:1052-0295
1473-7760
DOI:10.3109/10520295.2013.832799