Histological and immunohistochemical studies of the structure of lymph nodes in Kilis goats

Abstract Ten healthy adult Kilis goat mesenteric lymph nodes were used to examine the general structure of lymph nodes, lymphocytes, plasma cells, reticular cells and reticular fibers using histological methods. We also detected T lymphocytes using anti-CD3 [SP7], anti-CD4 [74-12-4], mouse anti-bovi...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2014-08, Vol.89 (6), p.440-445
Main Authors: Bozkurt, YA, Ku, S, Kozlu, T, Ba ak, F
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigens, CD - immunology
Goats
histology
Immunohistochemistry
Kilis goat
lymph node
Lymph Nodes - cytology
Mice
Paraffin Embedding - methods
T-Lymphocytes - cytology
T-Lymphocytes - immunology
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Summary:Abstract Ten healthy adult Kilis goat mesenteric lymph nodes were used to examine the general structure of lymph nodes, lymphocytes, plasma cells, reticular cells and reticular fibers using histological methods. We also detected T lymphocytes using anti-CD3 [SP7], anti-CD4 [74-12-4], mouse anti-bovine CD4 [CC30] and mouse anti-bovine CD8 [CC63] monoclonal antibodies (mAb); and B lymphocytes using anti-CD79a [HM57] mAb, macrophages using anti-macrophage [MAC387] mAb and follicular dendritic cells using anti-S100 polyclonal antibody (pAb). The distribution of these cells also was studied. Although the primer antibodies we used for CD3, CD8, CD79a, MAC387 and S100 worked well, the primer antibodies for CD4 were ineffective for paraffin embedded goat lymph nodes.
ISSN:1052-0295
1473-7760
DOI:10.3109/10520295.2014.889852