Evaluation of gene polymorphisms in exercise-induced oxidative stress and damage

Abstract Many potentially significant genetic variants related to oxidative stress have been identified and performance in endurance sports is a multi-factorial phenotype. Thus, it was decided to investigate the influences of the haptoglobin (Hp), MnSOD (Val9Ala), CAT (21A/T), GPX1 (Pro198Leu), ACE,...

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Published in:Free radical research 2010, Vol.44 (3), p.322-331
Main Authors: Akimoto, Arthur Kenji, Miranda-Vilela, Ana Luisa, Alves, Penha Cristina Zaidan, Pereira, Luiz Carlos da Silva, Lordelo, Graciana Souza, Hiragi, Cassia de Oliveira, da Silva, Izabel Cristina Rodrigues, Grisolia, Cesar Koppe, Klautau-Guimarães, Maria de Nazaré
Format: Article
Language:English
Subjects:
Adolescent
Adult
Alanine Transaminase - blood
antioxidant enzyme polymorphisms
Aspartate Aminotransferases - blood
Athletes
biochemical markers of tissue injuries (AST, ALT, CK)
Catalase - genetics
Comet Assay
Creatine Kinase - blood
DNA Damage - genetics
Exercise-induced damage
Female
Genetic Predisposition to Disease - genetics
Genotype
Glutathione Transferase - genetics
Haptoglobins - genetics
Humans
Lipid Peroxidation - genetics
Male
Middle Aged
Oxidative Stress - genetics
Peptidyl-Dipeptidase A - genetics
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Running - physiology
Superoxide Dismutase - genetics
TBARS assay
Young Adult
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Summary:Abstract Many potentially significant genetic variants related to oxidative stress have been identified and performance in endurance sports is a multi-factorial phenotype. Thus, it was decided to investigate the influences of the haptoglobin (Hp), MnSOD (Val9Ala), CAT (21A/T), GPX1 (Pro198Leu), ACE, glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) genes' polymorphisms on the oxidative stress and damage suffered by human athletes (runners). Blood samples taken immediately after a race were submitted to genotyping, comet and TBARS assays, biochemical analyses of creatine kinase (CK), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). MnSOD significantly influenced results of CK and a possible association between Hp1F-1S and Hp1S-2 genotypes with a superior TBARS values was found. Higher or lower TBARS and CK values or DNA damage also depended on the interaction between Hp and ACE or GST genotypes, indicating that MnSOD and Hp polymorphisms can be determining factors in performance, at least for runners.
ISSN:1071-5762
1029-2470
DOI:10.3109/10715760903494176