Quantification of ultraviolet radiation-induced DNA damage in the urine of Swedish adults and children following exposure to sunlight

Context: DNA damage following exposure to ultraviolet radiation (UVR) is important in skin cancer development. The predominant photoproduct, cyclobutane thymine dimer (T=T), is repaired and excreted in the urine, where it provides a biomarker of exposure. Objective: To quantify urinary T=T levels af...

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Bibliographic Details
Published in:Biomarkers 2012-11, Vol.17 (7), p.634-641
Main Authors: Liljendahl, Tove Sandberg, Kotova, Natalia, Segerbäck, Dan
Format: Article
Language:English
Subjects:
Adult
Biomarkers - urine
Child
Child, Preschool
DNA Damage
Environmental Exposure
Female
Humans
Male
Medicin och hälsovetenskap
Middle Aged
P-32-postlabelling
P-postlabelling
Pyrimidine Dimers - urine
Risk Factors
Skin Neoplasms - etiology
Statistics, Nonparametric
Sunbathing
sunlight
Sunlight - adverse effects
Sweden
thymine dimer
ultraviolet radiation
Ultraviolet Rays - adverse effects
urine
Young Adult
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Summary:Context: DNA damage following exposure to ultraviolet radiation (UVR) is important in skin cancer development. The predominant photoproduct, cyclobutane thymine dimer (T=T), is repaired and excreted in the urine, where it provides a biomarker of exposure. Objective: To quantify urinary T=T levels after recreational sunlight exposure in adults and children. Methods: Average UVR doses were measured with personal dosimeters. Urinary T=T was analysed with 32P-postlabelling. Results: Background levels of T=T increased significantly following exposure to sunlight. Amounts of T=T in urine of children and adults were not significantly different after adjusting for area of skin exposed and physiological differences. UVR dose and amounts of T=T correlated for both adults and children. Conclusion: Recreational exposure to sunlight in Sweden induces levels of DNA damage, clearly detectable in urine.
ISSN:1354-750X
1366-5804
1366-5804
DOI:10.3109/1354750X.2012.709881