Comparison of oral and transdermal administration of rasagiline mesylate on human melanoma tumor growth in vivo

• Published in: Cutaneous and ocular toxicology 2012-12, Vol.31 (4), p.312-317
• Main Authors: Meier-Davis, Susan R., Dines, Kevin, Arjmand, Fatima M., Hamlin, Richard, Huang, Betsy, Wen, Jainye, Christianson, Chad, Shudo, Jutaro, Nagata, Tetsuto
• Format: Article
• Language: English
• Subjects: Administration, Cutaneous; Administration, Oral; Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - blood; Antineoplastic Agents - pharmacokinetics; Disease Models, Animal; Female; Humans; Indans - administration & dosage; Indans - blood; Indans - pharmacokinetics; MAO-B; Melanin; Melanoma - drug therapy; Melanoma - metabolism; Melanoma - pathology; Mice; Mice, Nude; monoamine oxidase; Monoamine Oxidase Inhibitors - administration & dosage; Monoamine Oxidase Inhibitors - blood; Monoamine Oxidase Inhibitors - pharmacokinetics; patch; Skin Neoplasms - drug therapy; Skin Neoplasms - metabolism; Skin Neoplasms - pathology; SKMEL28; Tissue Distribution; topical; transdermal; Transdermal Patch; Tumor Burden - drug effects; xenograft; Xenograft Model Antitumor Assays
• ISSN: 1556-9527; 1556-9535
• DOI: 10.3109/15569527.2012.676119

Background/Aims: Transdermal patch administration results in a locally high concentration of drug that induce local toxicity, including tumorogenicity. As a worst-case scenario for consequences of repeated application on neoplastic growth, the melanin-binding drug, rasagiline, was used in a transdermal formulation applied directly to a human-derived melanoma to determine the effects on tumor growth. Materials and Methods: Rasagiline mesylate was administered either orally or transdermally to athymic mice implanted with human melanoma (SKMEL28) to determine the effects on tumor growth and survival. Over a 21-day period, animals were administered daily oral gavage (15 mg/kg) or one or two rasagiline mesylate transdermal patches every 3 days. After the last dose administration, blood samples were collected to confirm drug exposure. Results: All animals from the untreated, vehicle and rasagiline groups survived to the end of the study; however, 7 out of the 10 cisplatin-treated animals died before the end of the study. Rasagiline mesylate dosed either via the oral or transdermal routes had comparable plasma exposure and, unexpectedly, significantly reduced absolute tumor volumes and tumor growth rates in the nude mouse SKMEL28 xenograft model. Conclusion: Transdermal delivery of melanin-binding rasagiline does not increase melanoma growth in the xenograft model. Because rasagiline decreases melanoma growth, it may be candidate for combination therapy for melanoma.