Cytotoxicity of 1-octyl-3-methylimidazolium chloride on Escherichia coli DH5α

Abstract The mechanisms of the cytotoxic effect of ionic liquid (IL) 1-octyl-3-methylimidazolium chloride ([C8mim][Cl]) on bacterial cells were studied. Bacterial cells were exposed to 0, 0.1, 1 and 10 mmol   L−1 [C8mim][Cl] for 10 h. The results showed that [C8mim][Cl] inhibited bacterial cell prol...

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Bibliographic Details
Published in:Toxin reviews 2014-09, Vol.33 (3), p.91-94
Main Authors: Jing, Changqin, Hu, Huanhuan, Guo, Menglong, Chen, Xu, Li, Tianhan
Format: Article
Language:English
Subjects:
Cell membrane permeability
Escherichia coli DH5α
ionic liquid
metabolic activity
proliferation and growth
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Summary:Abstract The mechanisms of the cytotoxic effect of ionic liquid (IL) 1-octyl-3-methylimidazolium chloride ([C8mim][Cl]) on bacterial cells were studied. Bacterial cells were exposed to 0, 0.1, 1 and 10 mmol   L−1 [C8mim][Cl] for 10 h. The results showed that [C8mim][Cl] inhibited bacterial cell proliferation in a dose-dependent manner. When IL concentrations reached 10 mmol L−1, almost no cell division was observed. [C8mim][Cl] treatment significantly reduced succinate dehydrogenase activity. The amount of total protein significantly decreased with increasing [C8mim][Cl] concentrations of up to 10 mmol L−1. [C8mim][Cl] increased protein and nucleic acid contents in the extracellular fluid and increased cell permeability. The results suggest that the increase in cell membrane permeability, which was induced by [C8mim][Cl], could inhibit cell proliferation and metabolic activity in bacteria.
ISSN:1556-9543
1556-9551
DOI:10.3109/15569543.2013.867885