A comparison of six major platelet functional tests to assess the impact of carbon nanomaterials on platelet function: A practical guide

Abstract The study of the haemocompatibility of nanomaterials that could be in contact with blood (e.g. nanoparticle (NP)-based drug-delivery system) is of major importance. The primary objective of this study was to compare the ability of six platelet functional tests to assess the impact of NPs on...

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Bibliographic Details
Published in:Nanotoxicology 2014-03, Vol.8 (2), p.220-232
Main Authors: Laloy, Julie, Mullier, François, Alpan, Lutfiye, Mejia, Jorge, Lucas, Stéphane, Chatelain, Bernard, Toussaint, Olivier, Masereel, Bernard, Rolin, Stéphanie, Dogné, Jean-Michel
Format: Article
Language:English
Subjects:
blood
Blood Platelets - cytology
Blood Platelets - drug effects
Blood Platelets - metabolism
Carbon - toxicity
haemocompatibility
Humans
Impact-R
nanoparticles
Nanostructures - toxicity
Platelet Function Tests - methods
platelets
Toxicity Tests - methods
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Summary:Abstract The study of the haemocompatibility of nanomaterials that could be in contact with blood (e.g. nanoparticle (NP)-based drug-delivery system) is of major importance. The primary objective of this study was to compare the ability of six platelet functional tests to assess the impact of NPs on platelet function. The secondary objective was to determine an accurate and reliable screening test to measure the potential impact of NPs on primary haemostasis whatever their physicochemical properties. Four types of carbon NPs (carbon black, fullerenes, single-walled carbon nanotubes and multi-walled carbon nanotubes) were investigated on six platelet function tests: light transmission aggregometry, whole-blood impedance aggregometry, platelet function analyser-100 (PFA-100®) and Cone-and-Plate(let) analyser (Impact-R®), transmission- and field emission gun scanning electron microscopy (FEG-SEM). We considered that Impact-R® supported by FEG-SEM is the reference method to investigate the potential impact of NPs on platelet function.
ISSN:1743-5390
1743-5404
DOI:10.3109/17435390.2013.788750