Pseudomonas-Based Amperometric Detection of Biphenyl and Chlorinated Benzoates

Pseudomonas cells able to degrade biphenyl and chlorinated benzoates were used as a basis of the recognition element in amperometric biosensors. The possibility of detecting biphenyl and chlorinated benzoates in a range of concentrations from 0.002 to 0.3 mM and 0.01 to 1.0 mM, respectively, was sho...

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Bibliographic Details
Published in:Analytical letters 1999-01, Vol.32 (1), p.11-23
Main Authors: Reshetilov, A.N., Iliasov, P.V., Slepenkin, A.V., Grechkina, G.M., Starovoitov, I.I.
Format: Article
Language:English
Subjects:
amperometric detection
biphenyl
chlorinated benzoates
Pseudomonas strains
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Summary:Pseudomonas cells able to degrade biphenyl and chlorinated benzoates were used as a basis of the recognition element in amperometric biosensors. The possibility of detecting biphenyl and chlorinated benzoates in a range of concentrations from 0.002 to 0.3 mM and 0.01 to 1.0 mM, respectively, was shown. The sensors were characterized by wide substrate specificity. Their parameters and the dependence of signals on the measurement conditions (pH of the medium, temperature, salt concentration) were studied. It was suggested that these analyzers can be used in practice for evaluation of the total pool of aromatic xenobiotics available at high concentrations in chemical industry wastes.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719908542595