COMPARISON OF OXIDATIVE STRESS INDICATORS IN PLASMA OF RECENT-ONSET AND LONG-TERM TYPE 1 DIABETIC PATIENTS

• Published in: Journal of Toxicology and Environmental Health, Part A Part A, 2000-01, Vol.59 (1), p.7-14
• Main Authors: GÜZEL, S, SEVEN, A, SATMAN, I, BURCAK, G
• Format: Article
• Language: English
• Subjects: Adult; Aging - metabolism; Albuminuria - urine; Biological and medical sciences; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - complications; Diabetes. Impaired glucose tolerance; Diabetic Nephropathies - blood; Diabetic Neuropathies - blood; Diabetic Retinopathy - blood; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Humans; Lipid Peroxides - metabolism; Lipids - blood; Male; Medical sciences; Oxidative Stress - physiology; Phospholipids - blood; Thiobarbituric Acid Reactive Substances - metabolism; Time Factors
• ISSN: 1528-7394; 0098-4108; 1087-2620
• DOI: 10.1080/009841000157032

Oxidative stress was compared in plasm a of 15 recently diagnosed (5 yr) type 1 diabetic patients with 15 healthy volunteers. Lipid peroxidation indices measured in plasma included thiobarbituric acid-reactive substances (TBARS), conjugated dienes, and lipid hydroperoxide (ROOH). The values obtained were corrected for phospholipid to minimize this as a confounding factor. In recently diagnosed diabetics, plasm a conjugated lipid dienes were significantly elevated. However, in longstanding diabetics there was a marked increase in TBARS, conjugated dienes, and lipid hydroperoxide levels. Our findings showed increased oxidative stress in type 1 diabetics regardless of metabolic control and that conjugated diene measurement appeared to be the most sensitive bioindicator of oxidant stress in our population.