Dietary Cooked Navy Beans and Their Fractions Attenuate Colon Carcinogenesis in Azoxymethane-Induced Ob/Ob Mice

• Published in: Nutrition and cancer 2008-01, Vol.60 (3), p.373-381
• Main Authors: Bobe, G, Barrett, K.G, Mentor-Marcel, R.A, Saffiotti, U, Young, M.R, Colburn, N.H, Albert, P.S, Bennink, M.R, Lanza, E
• Format: Article
• Language: English
• Subjects: adenocarcinoma; Adenocarcinoma - chemically induced; Adenocarcinoma - prevention & control; adenoma; Adenoma - chemically induced; Adenoma - prevention & control; animal diseases; Animals; anticarcinogenic activity; Anticarcinogenic Agents - analysis; Anticarcinogenic Agents - pharmacology; azoxymethane; Azoxymethane - antagonists & inhibitors; Azoxymethane - toxicity; Biological and medical sciences; carcinogenesis; carcinogens; Carcinogens - antagonists & inhibitors; Carcinogens - toxicity; Colonic Neoplasms - chemically induced; Colonic Neoplasms - prevention & control; colorectal neoplasms; cooked foods; disaccharides; Fabaceae - chemistry; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Male; Medical sciences; Mice; Mice, Obese; monosaccharides; navy beans; nutritional intervention; nutritive value; oligosaccharides; phenolic compounds; Plant Extracts - analysis; Plant Extracts - pharmacology; plant residues; Random Allocation; saponins; Solubility; Tumors; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-5581; 1532-7914
• DOI: 10.1080/01635580701775142

Based on the protective effects of cooked dry bean consumption in a human intervention study, we evaluated which fraction of cooked dry beans is responsible for its cancer-preventive effects. Cooked navy beans (whole beans), the insoluble fraction (bean residue) or soluble fraction of the 60% (vol:vol) ethanol extract of cooked navy beans (bean extract), or a modified AIN-93G diet (16.6% fat including 12.9% lard) as control diet were fed to 160 male obese ob/ob mice after 2 azoxymethane injections. In comparison to control-fed mice, dysplasia, adenomas, or adenocarcinomas were detected in fewer mice on either bean fraction diet (percent reduction from control: whole beans 54%, P = 0.10; bean residue 81%, P = 0.003; bean extract 91%, P = 0.007), and any type of colon lesions, including focal hyperplasia, were found in fewer mice on each of the 3 bean diets percent reduction from control: whole bean 56%, P = 0.04; bean residue 67%, P = 0.01; bean extract 87%, P = 0.0003. These results suggest that both the soluble and the insoluble fraction of the extract contribute to the cancer-protective effect of cooked navy beans.