Elevated Levels of Human α -Defensin in Tears of Patients with Allergic Conjunctival Disease Complicated by Corneal Lesions: Detection by SELDI ProteinChip System and Quantification

• Published in: Current eye research 2005, Vol.30 (9), p.737-744
• Main Authors: Hida, Richard Y., Ohashi, Yoshiki, Takano, Yoji, Dogru, Murat, Goto, Eiki, Fujishima, Hiroshi, Saito, Ichiro, Saito, Kenji, Fukase, Yu, Tsubota, Kazuo
• Format: Article
• Language: English
• Subjects: alpha-Defensins - metabolism; atopic keratoconjunctivitis; Conjunctivitis, Allergic - complications; Conjunctivitis, Allergic - metabolism; Corneal Diseases - complications; Corneal Diseases - metabolism; Enzyme-Linked Immunosorbent Assay - methods; Eye Proteins - metabolism; Humans; Protein Array Analysis - methods; protein chip array; surface enhanced laser desorption ionization (SELDI); Tears - metabolism; α -defensin
• ISSN: 0271-3683; 1460-2202
• DOI: 10.1080/02713680591005986

Purpose: To analyze levels of α -defensin in the tears of allergic patients (with/without corneal lesions) comparing the results with those of normal control subjects. Methods: Screening of the protein profiles of the tears of allergic patients with corneal epithelial lesions and normal controls was performed by surface enhanced laser desorption/ionization (SELDI) ProteinChip array initially. ELISA was then performed to quantify the levels of α -defensin in the tears of allergic patients (with/without corneal epithelial lesions) and normal control patients. Results: Proteins expressing significant differences between patients and controls by SELDI analysis were examined. Several peptides with molecular weights similar to α -defensins were found to be expressed to a greater extent in allergic patients. ELISA was performed in tears of allergic patients and control subjects to ascertain the presence and increased expression of α -defensins in allergic patients. Concentrations of α -defensins in allergic patients with corneal epithelial lesions were significantly higher than those of allergic patients without epithelial lesions or normal controls. Conclusions: Alpha-defensins were found in greater concentrations in tears of allergic patients with corneal lesions. The antimicrobial effects of α -defensins may play a role in the prevention of secondary infection of corneal lesions in allergic patients. SELDI ProteinChip technology is a useful and effective tool in profiling the differential expression of proteins in tears.