PCR-based detection of Phomopsis azadirachtae in die-back affected neem seeds

Phomopsis azadirachtae Sateesh, Bhat & Devaki is the incitant of die-back disease of neem trees. Delayed appearance of conidia and presence of other microorganisms in the neem tissues are the obstacles in the rapid and accurate identification of P. azadirachtae. This work was carried out to deve...

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Bibliographic Details
Published in:Archiv für Phytopathologie und Pflanzenschutz 2009-07, Vol.42 (7), p.626-632
Main Authors: Girish, K., Bhat, S. Shankara, Raveesha, K. A.
Format: Article
Language:English
Subjects:
Die-back
neem
P. azadirachtae
PCR detection
Phomopsis
rDNA
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Summary:Phomopsis azadirachtae Sateesh, Bhat & Devaki is the incitant of die-back disease of neem trees. Delayed appearance of conidia and presence of other microorganisms in the neem tissues are the obstacles in the rapid and accurate identification of P. azadirachtae. This work was carried out to develop a methodology for rapid detection of the pathogen in diseased tissues especially in the neem seeds. rDNA sequences of many Phomopsis spp. were retrieved from the database and were subjected for multiple alignment to select a 179 bp conserved sequence. This was used to design Phomopsis specific primer pair (Forward and Reverse) having the potential to produce a 154 bp product in PCR. The primer pair was utilised to detect the presence of P. azadirachtae in diseased neem seeds and other tissues. This is the first report on the PCR-based detection of P. azadirachtae directly in die-back diseased neem tissues. This method can be employed for rapid and reliable detection of P. azadirachtae in die-back affected neem seeds. Hence it will have very good application in seed health testing laboratories.
ISSN:0323-5408
1477-2906
DOI:10.1080/03235400701287628