A standardized methodology for the study of induced glycosylated plant phenolics

Based on previously reported techniques and methods to identify induced phenolic compounds, a shorter, more reliable methodology for identifying conjugated plant phenolics from cucumber was developed. During the initial analytical phase of this methodology, compounds are elicited, extracted, hydroly...

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Bibliographic Details
Published in:Canadian journal of plant pathology 2002-12, Vol.24 (4), p.429-436
Main Authors: McNally, D.J., Wurms, K., Labbé, C., Bélanger, R.R.
Format: Article
Language:English
Subjects:
acides phénoliques
composés phénoliques glycosylés induits
Cucumis sativus
flavonoïde C-glycosides
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Summary:Based on previously reported techniques and methods to identify induced phenolic compounds, a shorter, more reliable methodology for identifying conjugated plant phenolics from cucumber was developed. During the initial analytical phase of this methodology, compounds are elicited, extracted, hydrolyzed, and fractionated. During the second semipreparative phase, scale-up chromatography is used to semipurify large amounts of extracted hydrolyzed induced compound, which are later purified with semipreparative high performance liquid chromatography. To demonstrate the merits of this methodology and the problematic nature of these defence molecules, comparisons are made with the methodologies of previous studies examining phytoalexin production in cucumber plants.Key words: Cucumis sativus, induced glycosylated phenolics, phenolic acids, C-flavonoid glycosides, phytoalexins, methodology.
ISSN:0706-0661
1715-2992
DOI:10.1080/07060660209507030