Nanofiltration for Purification of Nucleotide Sugars

Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of carbohydrate chemistry 1999-01, Vol.18 (1), p.41-49
Main Authors: Dudziak, Gregor, Fey, Sven, Hasbach, Lutz, Kragl, Udo
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu5Ac was 0.995 and for GDP-Man 0.983. Both nucleotide sugars were purified on a gram scale with a purity ≥95%.
ISSN:0732-8303
1532-2327
DOI:10.1080/07328309908543977