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Nanofiltration for Purification of Nucleotide Sugars
Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu...
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Published in: | Journal of carbohydrate chemistry 1999-01, Vol.18 (1), p.41-49 |
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Language: | English |
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cited_by | cdi_FETCH-LOGICAL-c344t-354b0bc283997c9d58946a5ea901f469d92b87cfc069127eade25630295809913 |
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cites | cdi_FETCH-LOGICAL-c344t-354b0bc283997c9d58946a5ea901f469d92b87cfc069127eade25630295809913 |
container_end_page | 49 |
container_issue | 1 |
container_start_page | 41 |
container_title | Journal of carbohydrate chemistry |
container_volume | 18 |
creator | Dudziak, Gregor Fey, Sven Hasbach, Lutz Kragl, Udo |
description | Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu5Ac was 0.995 and for GDP-Man 0.983. Both nucleotide sugars were purified on a gram scale with a purity ≥95%. |
doi_str_mv | 10.1080/07328309908543977 |
format | article |
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source | Taylor and Francis Science and Technology Collection |
title | Nanofiltration for Purification of Nucleotide Sugars |
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