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Nanofiltration for Purification of Nucleotide Sugars

Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu...

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Published in:Journal of carbohydrate chemistry 1999-01, Vol.18 (1), p.41-49
Main Authors: Dudziak, Gregor, Fey, Sven, Hasbach, Lutz, Kragl, Udo
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Language:English
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container_title Journal of carbohydrate chemistry
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creator Dudziak, Gregor
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description Nanofiltration was used in downstream processing for the desalination and concentration of the nucleotide sugars, cytidine-5'-monophospho-β-N-acetylneuraminic acid (CMP-Neu5Ac) 1 and guanosine-5'-diphospho-α-D-mannose (GDP-Man) 2. With appropriate membrances, the retention rate for CMP-Neu5Ac was 0.995 and for GDP-Man 0.983. Both nucleotide sugars were purified on a gram scale with a purity ≥95%.
doi_str_mv 10.1080/07328309908543977
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title Nanofiltration for Purification of Nucleotide Sugars
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