Differential expression of claudins in human endometrium and endometriosis

Membrane proteins of the claudin superfamily are important components of cellular tight and adherens junctions. Although their exact function remains unclear, these proteins may play a role in tissue remodeling, a process which is associated with several diseases including endometriosis. In the pres...

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Bibliographic Details
Published in:Gynecological endocrinology 2008-01, Vol.24 (8), p.442-449
Main Authors: Gaetje, Regine, Holtrich, Uwe, Engels, Knut, Kissler, Stefan, Rody, Achim, Karn, Thomas, Kaufmann, Manfred
Format: Article
Language:English
Subjects:
Adult
Claudin-1
claudins
Endometriosis
Endometriosis - genetics
Endometriosis - metabolism
Endometrium
Endometrium - metabolism
Female
Gene Expression Profiling
Gene Expression Regulation
Humans
Membrane Proteins - genetics
Membrane Proteins - metabolism
Menstrual Cycle - genetics
Menstrual Cycle - metabolism
microarray
Middle Aged
Multigene Family
Oligonucleotide Array Sequence Analysis
Peritoneal Diseases - genetics
Peritoneal Diseases - metabolism
Peritoneum - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Uterine Diseases - genetics
Uterine Diseases - metabolism
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Summary:Membrane proteins of the claudin superfamily are important components of cellular tight and adherens junctions. Although their exact function remains unclear, these proteins may play a role in tissue remodeling, a process which is associated with several diseases including endometriosis. In the present work we analyzed the expression of 13 members of the claudin family in the endometrium and peritoneum by microarray analysis. Real-time polymerase chain reaction and immunohistochemistry in human endometrium and peritoneal endometriotic lesions were performed for validation of the expression of claudin-1, -3, -4, -5 and -7. Diminished expression of claudin-3, -4 and -7 in ectopic endometrium was frequently observed as indicated by all three methods. In contrast to a higher expression of claudin-5 mRNA detected in bulk biopsies of ectopic endometrium, immunohistochemistry revealed no alteration of claudin-5 protein expression in glandular cells of endometriosis samples. The downregulation of various members of the claudin family may contribute to endometrial cell detachment and increase the number of cells invading pelvic organs.
ISSN:0951-3590
1473-0766
DOI:10.1080/09513590802242694