Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes

Purpose: To study the frequency of true incomplete exchanges in radiation-induced chromosome aberrations. Materials and methods: Human lymphocytes were exposed to 2 Gy and 5 Gy of gamma-rays. Chromosome aberrations were studied using the fluorescence in situ hybridization (FISH) technique with whole...

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Bibliographic Details
Published in:International journal of radiation biology 1998-05, Vol.73 (5), p.521-527
Main Authors: Wu, H., George, K., Yang, T. C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological effects of radiation
Chromosome Aberrations
Chromosomes, Human - radiation effects
DNA Probes
Female
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization, Fluorescence - methods
Ionizing radiations
Life Sciences (General)
Lymphocytes - radiation effects
Lymphocytes - ultrastructure
Space life sciences
Telomere
Tissues, organs and organisms biophysics
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Summary:Purpose: To study the frequency of true incomplete exchanges in radiation-induced chromosome aberrations. Materials and methods: Human lymphocytes were exposed to 2 Gy and 5 Gy of gamma-rays. Chromosome aberrations were studied using the fluorescence in situ hybridization (FISH) technique with whole chromosome-specific probes, together with human telomere probes. Chromosomes 2 and 4 were chosen in the present study. Results: The percentage of incomplete exchanges was 27% when telomere signals were not considered. After excluding false incomplete exchanges identified by the telomere signals, the percentage of incomplete exchanges decreased to 11%. Since telomere signals appear on about 82% of the telomeres, the percentage of true incomplete exchanges should be even lower and was estimated to be 3%. This percentage was similar for chromosomes 2 and 4 and for doses of both 2 Gy and 5 Gy. Conclusions: The percentage of true incomplete exchanges is significantly lower in gamma-irradiated human lymphocytes than the frequencies reported in the literature.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530098142068