An improved cage system for the bioassay of Metarhizium anisopliae on Thrips tabaci (Thysanoptera: Thripidae)

The ability of thrips and other minute insects to escape from conventional assay cups led to development of a successful assay chamber. In the present study, we evaluated three bioassay systems for thrips; Tashiro cage, microtube assay and leaf sandwich assay and introduced an improved thrips entomo...

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Bibliographic Details
Published in:Biocontrol science and technology 2008-01, Vol.18 (7), p.745-752
Main Authors: Pourian, Hamid-Reza, Ezzati-Tabrizi, Reyhaneh, Talaei-Hassanloui, Reza
Format: Article
Language:English
Subjects:
bioassay chamber
entomopathogenic fungus
escape
Metarhizium anisopliae
Thripidae
Thrips tabaci
Thysanoptera
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Summary:The ability of thrips and other minute insects to escape from conventional assay cups led to development of a successful assay chamber. In the present study, we evaluated three bioassay systems for thrips; Tashiro cage, microtube assay and leaf sandwich assay and introduced an improved thrips entomopathogenic bioassay system (TEBS). Our objective was to investigate the effect of Metarhizium anisopliae on Thrips tabaci using an easy-to-use and inexpensive holding chamber that would produce bioassay results with acceptable levels of accuracy and precision. In our assays, we found that escape of second-instar larvae from TEBS was significantly less than those of other methods which we tested. Our results indicated that our assay system was more suitable than the other conventional assays for the bioassay of entomopathogenic fungi on T. tabaci.
ISSN:0958-3157
1360-0478
DOI:10.1080/09583150802290618