Conversion of Soybean Oil to Biodiesel Fuel Using Lipozyme TL IM in a Solvent-free Medium

The conversion of soybean oil to biodiesel fuel was investigated in the presence of a lipase from Thermomyces lanuginosus (commercially called Lipozyme TL IM) in a solvent-free medium. The lipase was inactivated when more than 1.5 molar equivalent of methanol was added to the oil mixture. To fully c...

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Bibliographic Details
Published in:Biocatalysis and biotransformation 2004-01, Vol.22 (1), p.45-48
Main Author: Xu, Wei Du, Jing Zeng and Dehua Liu, Yuanyuan
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biodiesel
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Glycerol
Lipozyme
Methanolysis
Methods. Procedures. Technologies
Thermomyces lanuginosus
Transesterification
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Summary:The conversion of soybean oil to biodiesel fuel was investigated in the presence of a lipase from Thermomyces lanuginosus (commercially called Lipozyme TL IM) in a solvent-free medium. The lipase was inactivated when more than 1.5 molar equivalent of methanol was added to the oil mixture. To fully convert the oil to its corresponding methyl esters, the reaction was performed successfully by a three-step addition of 1 molar equivalent of methanol and under the optimized conditions (40°C, 150 rpm, 10% enzyme quantity based on oil weight), the maximum methyl ester (ME) yield was 98% after 12 h reaction. By-product glycerol had a negative effect on enzymatic activity and iso-propanol was found to be effective for glycerol removal, in the presence of which lipase expressed relatively high activity and more than 94% of the ME yield was maintained after being used repeatedly for 15 batches.
ISSN:1024-2422
1029-2446
DOI:10.1080/10242420410001661222