Analysis and characterization of the transcriptional unit of a new Mytilus galloprovincialis (Mollusca: Bivalvia) hsp70 gene

We isolated, analysed and characterized the transcriptional unit of an hsp70 gene of Mytilus galloprovincialis (Mollusca: Bivalvia). Sequence analysis of a total of 3653 bp, revealed a 1914 bp ORF encoding for a 637-amino acid polypeptide that exhibits all the characteristic signatures of the cytopl...

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Bibliographic Details
Published in:DNA sequence 2005-02, Vol.16 (1), p.36-43
Main Authors: Kourtidis, Antonis, Scouras, Zacharias G.
Format: Article
Language:English
Subjects:
AJ585375
Amino Acid Sequence
Animals
Base Sequence
bivalves
Bivalvia - genetics
Chromosome Mapping
Cloning, Molecular
cytoplasmic
DNA - genetics
Evolution, Molecular
HSP70 family
HSP70 Heat-Shock Proteins - genetics
inducible
Molecular Sequence Data
mollusks
Mussel
Open Reading Frames
Phylogeny
Transcription, Genetic
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Summary:We isolated, analysed and characterized the transcriptional unit of an hsp70 gene of Mytilus galloprovincialis (Mollusca: Bivalvia). Sequence analysis of a total of 3653 bp, revealed a 1914 bp ORF encoding for a 637-amino acid polypeptide that exhibits all the characteristic signatures of the cytoplasmic members of the HSP70 family. The ORF is not interrupted by introns and its 5′-flanking region contains five putative heat shock elements (HSEs), both indicating for an inducible hsp70 gene. Furthermore, nucleotide and amino acid sequence alignments with HSP70 genes from several molluscan and non-molluscan species confirmed that the newly isolated gene, the first complete in the genus Mytilus and in the Mollusca phylum, is a cytoplasmic and inducible hsp70 gene.
ISSN:1042-5179
DOI:10.1080/10425170400024391