Aberrant pre-mRNA splicing of a highly conserved cell cycle regulator, CDC25C, in myelodysplastic syndromes

Alternative pre-mRNA splicing alters gene expression and protein function, and aberrant splicing patterns can be associated with neoplasia. The potential role of disordered RNA splicing in myelodysplastic syndrome (MDS) is unexplored. We analysed the splicing repertoire of CDC25C- a gene localised t...

Full description

Saved in:
Bibliographic Details
Published in:Leukemia & lymphoma 2008-01, Vol.49 (5), p.989-993
Main Authors: Caudill, Jonathan S., Porcher, Julie C, Steensma, David P
Format: Article
Language:English
Subjects:
5q-syndrome
Alternative splicing
Case-Control Studies
cdc25 Phosphatases - genetics
cdc25c
Cell Cycle
cell cycle regulator
Chromosomes, Human, Pair 5
Gene Expression Regulation, Neoplastic
Humans
MDS
Myelodysplastic Syndromes - genetics
Protein Isoforms
RNA Precursors
RNA Splicing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Alternative pre-mRNA splicing alters gene expression and protein function, and aberrant splicing patterns can be associated with neoplasia. The potential role of disordered RNA splicing in myelodysplastic syndrome (MDS) is unexplored. We analysed the splicing repertoire of CDC25C- a gene localised to chromosome 5q31 and encoding a cyclin cyclin-dependent-kinase regulatory phosphatase critical for cell cycle checkpoint control - in MDS, acute myeloid leukemia, chronic lymphocytic leukemia and healthy tissues. Five novel splicing isoforms were detected, and the splicing patterns were generally distinct in neoplastic samples compared with healthy controls. One of the novel isoforms, which we have termed CDC25C-6, occurred in 58% of the samples in our cohort. The results of this study suggest the possibility of aberrant splicing contributing to the phenotype in MDS and other haematologic malignancies.
ISSN:1042-8194
1029-2403
DOI:10.1080/10428190801971690