Expression of NADPH oxidase homologues and accessory genes in human cancer cell lines, tumours and adjacent normal tissues

The family of NADPH oxidase (NOX) genes produces reactive oxygen species (ROS) pivotal for both cell signalling and host defense. To investigate whether NOX and NOX accessory gene expression might be a factor common to specific human tumour types, this study measured the expression levels of NOX gen...

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Bibliographic Details
Published in:Free radical research 2009-01, Vol.43 (6), p.523-532
Main Authors: Juhasz, Agnes, Ge, Yun, Markel, Susan, Chiu, Alice, Matsumoto, Linda, van Balgooy, Josephus, Roy, Krishnendu, Doroshow, James H.
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Dual Oxidases
Gene Expression
human cancer
human tumour cell lines
Humans
hydrogen peroxide
NADPH oxidase (NOX)
NADPH Oxidases - genetics
Neoplasm Invasiveness - genetics
Neoplasms - genetics
Neoplasms - pathology
reactive oxygen species (ROS)
Reverse Transcriptase Polymerase Chain Reaction
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Summary:The family of NADPH oxidase (NOX) genes produces reactive oxygen species (ROS) pivotal for both cell signalling and host defense. To investigate whether NOX and NOX accessory gene expression might be a factor common to specific human tumour types, this study measured the expression levels of NOX genes 1-5, dual oxidase 1 and 2, as well as those of NOX accessory genes NoxO1, NoxA1, p47phox, p67phox and p22phox in human cancer cell lines and in tumour and adjacent normal tissue pairs by quantitative, real-time RT-PCR. The results demonstrate tumour-specific patterns of NOX gene expression that will inform further studies of the role of NOX activity in tumour cell invasion, growth factor response and proliferative potential.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760902918683