Two-Step Purification of Cordycepin from Cordyceps millitaris by High-Speed Countercurrent Chromatography

Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2009-01, Vol.32 (16), p.2417-2423
Main Authors: Ju, Xiuyun, Sun, Yong, Cao, Xiaoying, Jiang, Jihong, Zhang, Tianyou, Ito, Yoichiro
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cordycepin
Cordyceps millitaris
General pharmacology
High-speed countercurrent chromatography
HPLC
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:Cordycepin is successfully isolated and purified from Cordyceps millitaris in a two-step purification by high-speed countercurrent chromatography. Two solvent systems, ethyl acetate-1-butanol-water (3:2:5, v/v/v) and trichloromethane-methanol-1-butanol-water (2:1:0.25:1, v/v/v/v), were used for the two-step purification. The purity of the prepared cordycepin was 98.1% according to the high-performance liquid chromatographic analysis.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826070903188153