The C4 biochemical pathway, and the anatomy of lemongrass (Cymbopogon citratus (DC) Stapf.) cultivated in temperate climates

Leaf anatomy (light and transmission electron microscopy, immunogold localisation of Rubisco) and physiology (C 4 photosynthetic enzyme activities, CO 2 assimilation and stomatal conductance) were studied in lemongrass, Cymbopogon citratus (DC) Stapf., cultivated in temperate climates (northwestern...

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Published in:Plant biosystems 2003-01, Vol.137 (2), p.175-184
Main Authors: Bertea, Cinzia Margherita, Buffa, Giorgio, Camusso, Wanda, Bossi, Simone, Scannerini, Silvano, Maffei, Massimo, Tesio, Manuela, D'Agostino, Giovanni, Mucciarelli, Marco
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Language:English
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Cymbopogon citratus
leaf anatomy
Lemongrass
NADP-ME variant
photosynthetic enzyme activities
Rubisco immunolocalisation
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container_issue 2
container_start_page 175
container_title Plant biosystems
container_volume 137
creator Bertea, Cinzia Margherita
Buffa, Giorgio
Camusso, Wanda
Bossi, Simone
Scannerini, Silvano
Maffei, Massimo
Tesio, Manuela
D'Agostino, Giovanni
Mucciarelli, Marco
description Leaf anatomy (light and transmission electron microscopy, immunogold localisation of Rubisco) and physiology (C 4 photosynthetic enzyme activities, CO 2 assimilation and stomatal conductance) were studied in lemongrass, Cymbopogon citratus (DC) Stapf., cultivated in temperate climates (northwestern Italy). The leaf blade had an NADP-ME Kranz anatomy, with bundle sheath cells containing chloroplasts located in a centrifugal position. Dimorphic chloroplasts, agranal and starchy in the bundle sheath and granal starchless in the mesophyll cells, were also observed. Rubisco, as determined by immunolocalisation, occurred only in the bundle sheath chloroplasts. Pyruvate, orthophosphate dikinase (PPDK), NADP malic dehydrogenase (NADP-MDH), NADP malic enzyme (NADP-ME), PEP-carboxykinase (PCK), and NAD malic enzyme (NAD-ME) activities were also measured, indicating an NADP-ME C 4 photosynthetic pathway. Some kinetic properties and chemicalphysical parameters of lemongrass NADP-ME and NADP-MDH as well as CO 2 compensation point and stomatal conductance values confirmed these preliminary data. C. citratus appeared to be well adapted to the varying environmental conditions typical of temperate climates, by retaining high NADP-MDH and NADP-ME activities and a low CO 2 compensation point.
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subjects Cymbopogon citratus
leaf anatomy
Lemongrass
NADP-ME variant
photosynthetic enzyme activities
Rubisco immunolocalisation
title The C4 biochemical pathway, and the anatomy of lemongrass (Cymbopogon citratus (DC) Stapf.) cultivated in temperate climates
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