Production of cycloartane triterpene glycosides in shoot cultures of Astragalus sieberi DC

Shoot tips of the germinated seeds of Astragalus sieberi DC. were cultured on MS-medium supplemented with 0.1 mg L −1 each of naphthalene acetic acid (NAA) and benzyl adenine (BA) for establishment of shoot cultures. The effect of different concentrations of growth regulators on saponin content was...

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Bibliographic Details
Published in:Natural product research 2010-03, Vol.24 (5), p.416-422
Main Author: El-Mawla, A.M.A. Abd
Format: Article
Language:English
Subjects:
Astragalus Plant - chemistry
Astragalus Plant - drug effects
Astragalus Plant - metabolism
Astragalus sieberi DC
Mass Spectrometry
Nuclear Magnetic Resonance, Biomolecular
Plant Growth Regulators - pharmacology
Plant Shoots - chemistry
Plant Shoots - growth & development
Plant Shoots - metabolism
saponins
Saponins - biosynthesis
Saponins - chemistry
Saponins - isolation & purification
shoot cultures
Triterpenes - chemistry
Triterpenes - isolation & purification
Triterpenes - metabolism
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Summary:Shoot tips of the germinated seeds of Astragalus sieberi DC. were cultured on MS-medium supplemented with 0.1 mg L −1 each of naphthalene acetic acid (NAA) and benzyl adenine (BA) for establishment of shoot cultures. The effect of different concentrations of growth regulators on saponin content was studied and optimised. Saponin content was monitored by HPLC analysis. The most appropriate growth regulator with which to produce the highest content of saponins in shoot cultures was 0.5 mg L −1 each of NAA and BA. The isolated cycloastragenol-3-O-glucoside was identified by spectral analysis and compared with an authentic sample.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786410902975632