COMBINED EXPOSURE TO DEET (N,N-DIETHYL-m-TOLUAMIDE) AND PERMETHRIN-INDUCED RELEASE OF RAT BRAIN MITOCHONDRIAL CYTOCHROME c

The release of cytochrome c from the mitochondrial intermembrane space can induce apoptosis. The levels of mitochondrial cytochrome c in rat brain following a single dermal dose of 400 mg/kg of DEET, and of 1.3 mg/kg of permethrin, alone or in combination were determined. Rats were sacrificed at a t...

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Bibliographic Details
Published in:Journal of Toxicology and Environmental Health, Part A Part A, 2001-06, Vol.63 (4), p.243-252
Main Authors: Abu-Qare, Aqel W., Abou-Donia, Mohamed B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - drug effects
Brain - enzymology
Calibration
Chromatography, High Pressure Liquid
Cytochrome c Group - metabolism
DEET - pharmacology
Insect Repellents - pharmacology
Insecticides - pharmacology
Medical sciences
Mitochondria - drug effects
Mitochondria - enzymology
Permethrin
Pesticides, fertilizers and other agrochemicals toxicology
Pyrethrins - pharmacology
Rats
Rats, Sprague-Dawley
Spectrophotometry, Ultraviolet
Toxicology
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Summary:The release of cytochrome c from the mitochondrial intermembrane space can induce apoptosis. The levels of mitochondrial cytochrome c in rat brain following a single dermal dose of 400 mg/kg of DEET, and of 1.3 mg/kg of permethrin, alone or in combination were determined. Rats were sacrificed at a time interval of 0.5, 1, 2, 4, 8, 16, 24, 48, or 72 h after dosing. Brain mitochondria were isolated and the levels of cytochrome c were measured using reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Average percentage recovery of cytochrome c
ISSN:1528-7394
0098-4108
1087-2620
DOI:10.1080/15287390151143640