Flow-Injection Spectrophotometric Determination of Amoxcillin, Cephalexin, Ampicillin, and Cephradine in Pharmaceutical Formulations

Amoxcillin, cephalexin, ampicillin, and cephradine were determined spectrophotometrically in the pure form and in the pharmaceutical formulations by using flow-injection analysis (FIA). The method is based upon the hydrolysis of the drug under investigation in basic medium and then the reduction of...

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Bibliographic Details
Published in:Analytical letters 2004-12, Vol.37 (10), p.2099-2110
Main Author: Al-Momani, I. F.
Format: Article
Language:English
Subjects:
Amoxcillin
Ampicillin
Analytical chemistry
Cephalexin
Cephradine
Chemistry
Exact sciences and technology
Flow-injection analysis
Pharmaceutical analysis
Spectrometric and optical methods
Spectrophotometric
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Summary:Amoxcillin, cephalexin, ampicillin, and cephradine were determined spectrophotometrically in the pure form and in the pharmaceutical formulations by using flow-injection analysis (FIA). The method is based upon the hydrolysis of the drug under investigation in basic medium and then the reduction of the formed hydrolyzed product by the on-line generated I 2 in acidic medium. The decrease in the intensity of the I 2 color was monitored at 460 nm and found to be proportional to the concentration of the compound under investigation. Variables such as acidity, reagent concentrations, flow rate of reagents, and other FI parameters were optimized to produce the most sensitive and reproducible results. The method was successfully applied to the analysis of pharmaceutical preparations.
ISSN:0003-2719
1532-236X
DOI:10.1081/AL-200026683