Efficient extraction of paclitaxel and related taxoids from leaf tissue of Taxus using a potable solvent system

Leaves of Taxus x media and Taxus brevifolia were examined for their potential use as a source of paclitaxel and related taxoids. Various solvent systems were compared for their efficacy in the extraction of paclitaxel, as well as the extraction of contaminating residue. A method of extraction has b...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 1999, Vol.22 (11), p.1715-1732
Main Authors: Ketchum, R.E.B, Luong, J.V, Gibson, D.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
extraction
General pharmacology
leaves
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
solvents
Taxus brevifolia
Taxus media
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Leaves of Taxus x media and Taxus brevifolia were examined for their potential use as a source of paclitaxel and related taxoids. Various solvent systems were compared for their efficacy in the extraction of paclitaxel, as well as the extraction of contaminating residue. A method of extraction has been developed that enriches paclitaxel by over 500-fold using only potable solvents in combination with solid phase extraction, thus avoiding the use of chlorinated hydrocarbon solvent systems. This solvent system can be used on fresh leaf material, eliminating the need for drying material before extraction, and preventing degradation of the taxoids during the drying process. Using these methods, paclitaxel constituted 0.01 to 0.02% of the dry weight of the leaf tissue from Taxus x media, and 0.04% from dried leaves of Taxus brevifolia.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-100101762