SILVER ION HPLC OF p-METHOXYPHENACYL DERIVATIVES OF UNSATURATED FATTY ACIDS. III. MOBILE PHASE EFFECTS ON TRANS 6-, 9-, AND 11-18 : 1

• Published in: Journal of liquid chromatography & related technologies 2002-03, Vol.25 (4), p.615-625
• Main Authors: Momchilova, Sv, Nikolova-Damyanova, B.
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fat industries; Food industries; Fundamental and applied biological sciences. Psychology; Other chromatographic methods
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120008815

The effect of the mobile phase composition on the retention and resolution of trans positionally isomeric 6-, 9-, and 11-18 : 1 fatty acids was studied on a model mixture by silver ion high performance liquid chromatography (Ag-HPLC). Prior to chromatography, the fatty acids were converted into p-methoxyphenacyl derivatives. Hexane-based and dichloromethane-based mobile phases modified with either acetonitrile or methanol, or isopropanol were examined. The modifiers were found to have a different effect on the resolution, depending on the main solvent component in the mobile phase. In case of hexane being the main mobile phase solvent, best resolution was achieved when using isopropanol as a modifier. In dichloromethane-based mobile phases, best results were achieved with acetonitrile or methanol. The three trans isomers were completely resolved in practically all mobile phases tested, the elution order being 11-, 9-, 6-18 : 1, irrespective of the mobile phase composition.