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Simultaneous Determination of Retinoic Acid, Retinol, and Retinyl Palmitate in Ram Plasma by Liquid Chromatography

A liquid chromatographic method is described for the simultaneous determination of retinoic acid, retinol, and retinyl palmitate in ram plasma. Samples of 0.2 mL are extracted with 2-propanol-dichloromethane, the extracts are centrifuged, the supernatants are collected, evaporated, reconstituted in...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2003-03, Vol.26 (4), p.559-572
Main Authors: Moulas, A. N., Zervos, I. A., Taitzoglou, I. A., Tsantarliotou, M. P., Botsoglou, N. A.
Format: Article
Language:English
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Summary:A liquid chromatographic method is described for the simultaneous determination of retinoic acid, retinol, and retinyl palmitate in ram plasma. Samples of 0.2 mL are extracted with 2-propanol-dichloromethane, the extracts are centrifuged, the supernatants are collected, evaporated, reconstituted in mobile phase, and analyzed on a C 8 column using two consecutive isocratic elutions with methanol tetrahydrofuran-acetate buffer. Detection is performed at 350 and 325 nm using wavelength change during the run. The method exhibits analytical characteristics well within acceptable limits. Overall recoveries were 73.7% for retinoic acid, 90.2% for retinol, and 87.7% for retinyl palmitate. Precision values, expressed as % relative standard deviation, were in the range of 1.16-6.18%, while limits of detection were in the range of 0.3-10.0 ng/mL for all analytes.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-120017902