MOZPhCSE, a New Coumarin Based Fluorescent Derivatization Reagent

The synthesis of a new fluorescent probe 2-(3,7-dioxo-2-phenyl-3,7-dihydrochromeno[7,6-b][1,4]oxazin-9-yl)acetic acid succinimidyl ester was described. The reagent reacts easily with primary amines and forms highly fluorescent derivatives that can be separated and detected with an HPLC-fluorometric...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2004-01, Vol.27 (17), p.2699-2713
Main Authors: Gikas, Evagelos, Parissi-Poulou, Maria, Kazanis, Michael, Vavagianis, Andreas
Format: Article
Language:English
Subjects:
Amine
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Coumarin
Derivatization
Exact sciences and technology
Fluorescent reagent
MOZPhCSE
Other chromatographic methods
Oxazin-2-one
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Description
Summary:The synthesis of a new fluorescent probe 2-(3,7-dioxo-2-phenyl-3,7-dihydrochromeno[7,6-b][1,4]oxazin-9-yl)acetic acid succinimidyl ester was described. The reagent reacts easily with primary amines and forms highly fluorescent derivatives that can be separated and detected with an HPLC-fluorometric detector. The new reagent was evaluated using pentylamine as a model compound. The separation was achieved on a C 18 column using a mixture of acetonitrile:ammonium acetate buffer as the mobile phase. Statistical evaluation of the methodology described reveals good linearity, precision and accuracy, and high sensitivity. Application of the methodology can be used for the separation of four aliphatic amines (pentylamine, hexylamine, octylamine, and decylamine).
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-200029226