Combination of ion beam stabilisation, plasma etching and plasma deposition for the development of tissue engineering micropatterned supports

The performance of biomedical assays at both molecular and cellular level depends greatly on the ability to design new polymer surfaces. Patterns can be created by using materials with contrasted surface properties. In this work we describe in detail the preparation of micropatterned surfaces to be...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2004-01, Vol.15 (2), p.161-172
Main Authors: Manso, M., Rossini, P., Malerba, I., Valsesia, A., Gribaldo, L., Ceccone, G., Rossi, F.
Format: Article
Language:English
Subjects:
Acrylates - chemistry
Acrylates - radiation effects
ACRYLIC ACID
Animals
Biomedical Engineering
Cells, Cultured
ENDOTHELIAL CELLS
Humans
ION-BEAM STABILISATION
Microscopy, Atomic Force
Nanotechnology
PLASMA-ENHANCED CHEMICAL
POLY ETHYLENE GLYCOL
Polyethylene Glycols - chemistry
Polyethylene Glycols - radiation effects
Spectroscopy, Fourier Transform Infrared
Surface Properties
Surface-Active Agents - chemistry
VAPOUR DEPOSITION
X-RAY PHOTOELECTRON SPECTROSCOPY
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Summary:The performance of biomedical assays at both molecular and cellular level depends greatly on the ability to design new polymer surfaces. Patterns can be created by using materials with contrasted surface properties. In this work we describe in detail the preparation of micropatterned surfaces to be used as tissue engineering supports. Poly(ethylene glycol) (PEG) was used as the 'anti-fouling' polymer in opposition to functional regions covered by acrylic acid (AAc). Since spin-casted PEG films are unstable, ion beam stabilization (IBS) treatment was applied in order to render it insoluble. On the other hand, AAc films were deposited by low-power plasma chemical vapour deposition. Chemical properties of both polymers were monitored by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy while topographic features were followed by atomic force microscopy. Finally, a micropattern was produced by using a mask, which isolated the IBS-PEG from the AAc-deposited regions. Endothelial cells cultured on the surface were observed to follow the micropatterns. In fact, for a certain surface density it was observed that the cells present tensile or compressive stresses when forced to remain in the anti-fouling or the functionalised regions, respectively.
ISSN:0920-5063
1568-5624
DOI:10.1163/156856204322793557