Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering

Fibrous materials have morphological similarities to natural cartilage extracellular matrix and have been considered as candidate for bone tissue engineering scaffolds. In this study, we have evaluated a novel electrospun chitosan mat composed of oriented sub-micron fibers for its tensile property a...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2005-01, Vol.16 (7), p.861-873
Main Authors: Subramanian, Anuradha, Vu, David, Larsen, Gustavo F., Lin, Hsin-Yi
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
CARTILAGE REPAIR
Cartilage, Articular - cytology
Cell Adhesion - physiology
Cell Proliferation
Chitosan
CHITOSAN NANOFIBER
CHONDROCYTE
Chondrocytes - cytology
Chondrocytes - ultrastructure
Dogs
ELECTROSPINNING
Membranes, Artificial
Microscopy, Electron, Scanning
Polyethylene Glycols
Tensile Strength
TISSUE ENGINEERING
Tissue Engineering - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fibrous materials have morphological similarities to natural cartilage extracellular matrix and have been considered as candidate for bone tissue engineering scaffolds. In this study, we have evaluated a novel electrospun chitosan mat composed of oriented sub-micron fibers for its tensile property and biocompatibility with chondrocytes (cell attachment, proliferation and viability). Scanning electronic microscope images showed the fibers in the electrospun chitosan mats were indeed aligned and there was a slight cross-linking between the parent fibers. The electrospun mats have significantly higher elastic modulus (2.25 MPa) than the cast films (1.19 MPa). Viability of cells on the electrospun mat was 69% of the cells on tissue-culture polystyrene (TCP control) after three days in culture, which was slightly higher than that on the cast films (63% of the TCP control). Cells on the electrospun mat grew slowly the first week but the growth rate increased after that. By day 10, cell number on the electrospun mat was almost 82% that of TCP control, which was higher than that of cast films (56% of TCP). The electrospun chitosan mats have a higher Young's modulus (P
ISSN:0920-5063
1568-5624
DOI:10.1163/1568562054255682