The effect of PAMAM dendrimers on human and bovine serum albumin at different pH and NaCl concentrations

The effect of PAMAM G3.5, PAMAM G4 and PAMAM-OH G4 dendrimers on human and bovine serum albumins has been studied by fluorescence spectroscopy at different pH and ionic strength. It has been shown that the interactions between dendrimers and proteins depend on pH and the efficiency of interactions c...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2005-01, Vol.16 (9), p.1081-1093
Main Authors: Shcharbin, D., Klajnert, B., Bryszewska, M.
Format: Article
Language:English
Subjects:
Animals
Cattle
Dendrimers
FLUORESCENCE
Humans
Hydrogen-Ion Concentration
IONIC STRENGTH
Osmolar Concentration
PAMAM DENDRIMER
Polyamines - chemistry
Polyamines - pharmacology
Protein Conformation - drug effects
SERUM ALBUMIN
Serum Albumin - chemistry
Serum Albumin - drug effects
Sodium Chloride
Spectrometry, Fluorescence
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Summary:The effect of PAMAM G3.5, PAMAM G4 and PAMAM-OH G4 dendrimers on human and bovine serum albumins has been studied by fluorescence spectroscopy at different pH and ionic strength. It has been shown that the interactions between dendrimers and proteins depend on pH and the efficiency of interactions can be regulated by changing pH. The maximal pH dependence was observed for interactions between albumins and PAMAM G4 dendrimer. At physiological pH all dendrimers affect proteins in the maximum degree. Dendrimers had no effect on N-F and N-B transitions of albumins. The effect of dendrimers on HSA was smaller than for BSA. The increase of NaCl concentration led to a decrease of interactions between dendrimers and proteins.
ISSN:0920-5063
1568-5624
DOI:10.1163/1568562054798518