Effects of myasthenia gravis patients' sera with different autoantibodies on slow K+ current at mouse motor nerve terminals

The antibodies against pre-synaptic membrane receptor (PsmR) and acetylcholine receptor (AChR) in serum samples of myasthenia gravis (MG) patients and healthy donors were tested by enzyme-linked immunosorbent assays (ELISA). The serum samples of eight MG patients with different autoantibodies and th...

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Bibliographic Details
Published in:Neurological research (New York) 2003-01, Vol.25 (1), p.58-62
Main Authors: Xu, Tong-Hui, Ding, Jun, Shi, Yu-Liang, Li, Mu-Feng, Lu, Chuan-Zhen, Qiao, Jian
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Autoantibodies - immunology
Autoantibodies - pharmacology
Cells, Cultured
Child
Child, Preschool
Electrophysiology
Humans
MEMBRANE CURRENTS
Membrane Potentials - drug effects
Mice
Middle Aged
Motor Neurons - drug effects
Motor Neurons - physiology
MOUSE MOTOR NERVE TERMINALS
MYASTHENIA GRAVIS
Myasthenia Gravis - blood
Myasthenia Gravis - immunology
PERI-NEURIAL RECORDING
Potassium Channels - drug effects
Potassium Channels - physiology
POTASSIUM CURRENTS
PRE-SYNAPTIC MEMBRANE RECEPTOR ANTIBODY
Receptors, Presynaptic - drug effects
Receptors, Presynaptic - physiology
Synaptic Membranes - drug effects
Synaptic Membranes - physiology
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Summary:The antibodies against pre-synaptic membrane receptor (PsmR) and acetylcholine receptor (AChR) in serum samples of myasthenia gravis (MG) patients and healthy donors were tested by enzyme-linked immunosorbent assays (ELISA). The serum samples of eight MG patients with different autoantibodies and those of six healthy donors without these two kinds of autoantibodies were collected to investigate their effects on the peri-neurially recorded membrane currents at mouse motor nerve terminals. After inhibition of both fast and Ca 2+ -dependent K + currents by tetra-ethylammonium (TEA), a positive wave was revealed, which was a balance of the slow K + (I K,s ) and Ca 2+ currents (I Ca ). Application of anti-PsmR antibody negative MG sera and healthy donor sera, whether anti-AChR antibody positive or negative, did not affect the positive wave. However, the positive wave shifted to prolonged Ca 2+ -plateau when adding two of four anti-PsmR antibody positive serum samples from MG patients, indicating an inhibition of I K,s by anti-PsmR antibody positive sera. Meanwhile, all serum samples derived from either patients or healthy donors did not affect I Na .
ISSN:0161-6412
1743-1328
DOI:10.1179/016164103101200932