Down-regulation of Stat3 induces apoptosis of human glioma cell: a potential method to treat brain cancer

Objectives: Glioma is the most common brain tumor in central nervous system. Traditional therapies are not effective to cure this disease. Stat3 is a member of the signal transducer and activator of transcription family, and it has the potential to mediate cell survival, growth and differentiation....

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Published in:Neurological research (New York) 2008-04, Vol.30 (3), p.297-301
Main Authors: Ren, Wenwen, Duan, Yanhong, Yang, Yang, Ji, Yonghua, Chen, Fuxue
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Cell Line, Tumor
Down-Regulation - physiology
GLIOMA
Glioma - metabolism
Glioma - physiopathology
Green Fluorescent Proteins - biosynthesis
Humans
RNA Interference - physiology
RNAI
STAT3
STAT3 Transcription Factor - metabolism
Time Factors
Transfection
TUNEL
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Summary:Objectives: Glioma is the most common brain tumor in central nervous system. Traditional therapies are not effective to cure this disease. Stat3 is a member of the signal transducer and activator of transcription family, and it has the potential to mediate cell survival, growth and differentiation. Methods: In this study, we testified that Stat3 was constitutively expressed in glioma cell line SHG44 and then investigated the role of a low level of Stat3 expression in glioma cells by constructing an interfering RNA expression plasmid. Results: The results showed that glioma cells underwent morphologic and biochemical changes after the RNAi treatment. Discussion: We hypothesized that a low level of Stat3 expression could induce apoptosis of glioma cell, which further proved that Stat3 played an important role in growth, survival and proliferation of glioma cells. This study provides a new alternative to gene therapy for glioma treatment.
ISSN:0161-6412
1743-1328
DOI:10.1179/016164107X230784