Minimum concentration of cells required for the preparation of compact plasma-thrombin cell blocks

The compact plasma-thrombin technique is a simple and effective method for preparing cells for histopathology. However, the minimum cell suspension concentration necessary to prepare a clot of sufficient size and density has not been determined. A cell line of known concentration was serially dilute...

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Bibliographic Details
Published in:Journal of histotechnology 2012-07, Vol.35 (2), p.68-70
Main Authors: Cecchetelli, Alyssa, Kwan, Ricky, Krauter, Stephanie, Chiriboga, Luis
Format: Article
Language:English
Subjects:
Cell count
Cell culture
Plasma-thrombin clot
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Summary:The compact plasma-thrombin technique is a simple and effective method for preparing cells for histopathology. However, the minimum cell suspension concentration necessary to prepare a clot of sufficient size and density has not been determined. A cell line of known concentration was serially diluted in order to determine the minimum cell suspension concentration required to produce a clot of reasonable size and cell density.
ISSN:0147-8885
2046-0236
DOI:10.1179/2046023612Y.0000000007