Cloning and Transcriptional Analysis of the Gene Encoding 5-Aminolevulinic Acid Synthase of the White-Rot Fungus Phanerochaete sordida YK-624

In this study, we cloned the gene encoding 5-aminolevulinic acid synthase (ALAS) from the hyper-lignin-degrading fungus Phanerochaete sordida YK-624. The deduced amino acid sequence showed highest identity (93.0%) to ALAS of P. chrysosporium. Expression of the gene encoding ALAS, which we named aas,...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011-01, Vol.75 (1), p.178-180
Main Authors: MISUMI, Kenta, SUGIURA, Tatsuki, YAMAGUCHI, Shinya, MORI, Toshio, KAMEI, Ichiro, HIRAI, Hirofumi, KAWAGISHI, Hirokazu, KONDO, Ryuichiro
Format: Article
Language:English
Subjects:
5-Aminolevulinate Synthetase - chemistry
5-Aminolevulinate Synthetase - genetics
5-Aminolevulinate Synthetase - metabolism
5-aminolevulinic acid synthase
Amino Acid Sequence
Amino acids
Biochemistry
Biological and medical sciences
Biotechnology
Cloning
Cloning, Molecular
Encoding
Fundamental and applied biological sciences. Psychology
Fungi
gene cloning
Genes
Manganese
Molecular Sequence Data
Phanerochaete
Phanerochaete - enzymology
Phanerochaete - genetics
Phanerochaete chrysosporium
Phanerochaete sordida YK-624
Sequence Alignment
Transcription, Genetic
transcriptional analysis
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Summary:In this study, we cloned the gene encoding 5-aminolevulinic acid synthase (ALAS) from the hyper-lignin-degrading fungus Phanerochaete sordida YK-624. The deduced amino acid sequence showed highest identity (93.0%) to ALAS of P. chrysosporium. Expression of the gene encoding ALAS, which we named aas, corresponded temporally with the expression and activity of manganese peroxidase.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1271/bbb.100674