Effects of Peanut-Skin Procyanidin A1 on Degranulation of RBL-2H3 Cells

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (9), p.1644-1648
• Main Authors: TOMOCHIKA, Keisuke, SHIMIZU-IBUKA, Akiko, TAMURA, Tomoko, MURA, Kiyoshi, ABE, Naoki, ONOSE, Jun-ichi, ARAI, Soichi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Allergic Agents - chemistry; Anti-Allergic Agents - pharmacology; Anti-Allergic Agents - therapeutic use; Antiallergics; Arachis - chemistry; Arachis hypogaea; beta-N-Acetylhexosaminidases - analysis; beta-N-Acetylhexosaminidases - secretion; Biological and medical sciences; Calcium - metabolism; Catechin - chemistry; Catechin - pharmacology; Catechin - therapeutic use; Cell Degranulation - drug effects; Cell Degranulation - immunology; Cell Line, Tumor; degranulation; Fundamental and applied biological sciences. Psychology; Hydroquinones - antagonists & inhibitors; Hydroquinones - pharmacology; Hypersensitivity - drug therapy; Hypersensitivity - immunology; Hypersensitivity - prevention & control; Leukemia, Basophilic, Acute - immunology; Leukemia, Basophilic, Acute - metabolism; Leukemia, Basophilic, Acute - pathology; Magnetic Resonance Spectroscopy; peanut skin; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; polyphenol; Polyphenols - chemistry; Polyphenols - pharmacology; Polyphenols - therapeutic use; Proanthocyanidins - chemistry; Proanthocyanidins - pharmacology; Proanthocyanidins - therapeutic use; procyanidin; rat basophilic leukemia (RBL)-2H3; Rats; Seeds - chemistry; Signal Transduction - drug effects; Signal Transduction - immunology; Tetradecanoylphorbol Acetate - antagonists & inhibitors; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.110085

Peanut skin contains large amounts of polyphenols having antiallergic effects. We found that a peanut-skin extract (PSE) inhibits the degranulation induced by antigen stimulation of rat basophilic leukemia (RBL-2H3) cells. A low-molecular-weight fraction from PSE, PSEL, also had inhibitory activity against allergic degranulation. A main polyphenol in PSEL was purified by gel chromatography and fractionated by YMC-gel ODS-AQ 120S50 column. Electrospray ionization mass spectrometry (ESI-MS) analysis of the purified polyphenol gave m⁄z 599 [M+Na] + . Based on the results of 1 H-NMR, 13 C-NMR spectra, and optical rotation analysis, the polyphenol was identified as procyanidin A1. It inhibited the degranulation caused by antigen stimulation at the IC 50 of 20.3 μM. Phorbol-12-myristate-13-acetate (PMA) and 2,5,-di(tert-butyl)-1,4-hydroquinone (DTBHQ)-induced processes of degranulation were also inhibited by procyanidin A1. These results indicate that peanut-skin procyanidin A1 inhibits degranulation downstream of protein kinase C activation or Ca 2+ influx from an internal store in RBL-2H3 cells.