Immunohistochemical Localization of Endogenous D-Aspartate in the Marine Brown Alga Sargassum fusiforme

Immunohistochemical localization (cellular localization) of endogenous D-aspartate in the marine brown alga Sargassum fusiforme was investigated by the use of a specific polyclonal antibody raised against D-aspartate. D-Aspartate immunoreactivity was evident in the medullary layer in the blade of th...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (8), p.1481-1484
Main Authors: YOKOYAMA, Takehiko, AMANO, Masafumi, SEKINE, Masae, HOMMA, Hiroshi, TOKUDA, Masaharu, SATO, Minoru
Format: Article
Language:English
Subjects:
Animals
Antibodies - analysis
antibody
Aquatic Organisms - ultrastructure
Biological and medical sciences
brown alga (Phaeophyta)
Cytosol - chemistry
Cytosol - ultrastructure
D-amino acid
D-aspartate
D-Aspartic Acid - analysis
Female
Fundamental and applied biological sciences. Psychology
Immunoblotting
Immunohistochemistry
Japan
Microscopy
Rabbits
Sargassum - ultrastructure
Sargassum fusiforme (formerly Hizikia fusiformis)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immunohistochemical localization (cellular localization) of endogenous D-aspartate in the marine brown alga Sargassum fusiforme was investigated by the use of a specific polyclonal antibody raised against D-aspartate. D-Aspartate immunoreactivity was evident in the medullary layer in the blade of the alga, and weak staining was found in the cortical layer, whereas epidermal cells were found to lack D-aspartate. Within the cells of the layers, immunoreactivity was confirmed only in the cytosol and not in the cell wall, chloroplast, or vacuole. These results suggest that D-aspartate is present in S. fusiforme cells, and excludes the possibility that it is derived from attached or symbiotic organisms such as marine bacteria. This is the first report describing the localization of free D-aspartate in plant cells.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.110184