Mutants of Lactobacillus plantarum ML11-11 Deficient in Co-Aggregation with Yeast Exhibited Reduced Activities of Mixed-Species Biofilm Formation

Lactic acid bacteria (LAB) mutants deficient in inter-species co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decr...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2012, Vol.76 (2), p.326-330
Main Authors: FURUKAWA, Soichi, NOJIMA, Natsumi, NOZAKA, Soma, HIRAYAMA, Satoru, SATOH, Ayumi, OGIHARA, Hirokazu, MORINAGA, Yasushi
Format: Article
Language:English
Subjects:
Bacterial Proteins - analysis
Biofilms - growth & development
Biological and medical sciences
Cell Adhesion
co-aggregation
Coculture Techniques
Fundamental and applied biological sciences. Psychology
Lactobacillus plantarum
Lactobacillus plantarum - cytology
Lactobacillus plantarum - genetics
Membrane Proteins - analysis
mixed-species biofilm
Mutation
Proteomics
Saccharomyces cerevisiae
Saccharomycetales - cytology
Species Specificity
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Summary:Lactic acid bacteria (LAB) mutants deficient in inter-species co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decreases in mixed-species biofilm formation. These results suggest the important role of co-aggregation between LAB and yeast in mixed-species biofilm formation. Cell surface proteins obtained by 5 M LiCl extraction from the wild-type cells and non-co-aggregative mutant cells were analyzed by SDS-PAGE. There was an obvious difference in protein profiles. The protein band at 30 kDa was present abundantly in the wild-type cell surface fraction but was significantly decreased in the mutant cells. This band assuredly corresponded to the LAB surface factors that contribute to co-aggregation with yeasts.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.110714