Effect of dietary epigallocatechin-3-gallate on cytochrome P450 2E1-dependent alcoholic liver damage: Enhancement of fatty acid oxidation

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-12, Vol.71 (12), p.2999-3006
• Main Authors: Yun, J.W.(Amorepacific Corp. R and D Center, Yongin (Korea R.)), Kim, Y.K, Lee, B.S, Kim, C.W, Hyun, J.S, Baik, J.H, Kim, J.J, Kim, B.H
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - metabolism; ACIDE GRAS; ACIDOS GRASOS; ALANINA AMINOTRANSFERASA; ALANINE AMINOTRANSFERASE; alcohol; ALCOHOLES; ALCOHOLS; ALCOOL; Animals; Antioxidants - pharmacology; Antioxidants - therapeutic use; ASPARTATE AMINOTRANSFERASE; Aspartate Aminotransferases - metabolism; ASPARTATO AMINOTRANSFERASA; Biological and medical sciences; Carnitine O-Palmitoyltransferase - metabolism; carnitine palmitoyl-transferase 1 (CPT-1); CATECHIN; Catechin - analogs & derivatives; Catechin - pharmacology; Catechin - therapeutic use; CATECHINE; CATEQUINA; CITOCROMO P450; Cytochrome P-450 CYP2E1 - metabolism; CYTOCHROME P450; cytochrome P450 2E1 (CYP2E1); Enzyme Activation; epigallocatechin-3-gallate (EGCG); FATTY ACIDS; FATTY LIVER; Fatty Liver, Alcoholic - drug therapy; Fatty Liver, Alcoholic - metabolism; Fundamental and applied biological sciences. Psychology; HIGADO GRASO; Lipid Peroxidation - drug effects; Male; phospho-acetyl CoA carboxylase (p-ACC); Rats; Rats, Wistar; STEATOSE HEPATIQUE; TEA; THE
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70403

This study was designed to determine whether dietary epigallocatechin-3-gallate (EGCG), the most abundant catechin polyphenol in green tea, can protect the liver from cytochrome P450 2E1 (CYP2E1)-dependent alcoholic liver damage. Compared with an ethanol group, when EGCG was present in the ethanol diet, the formation of a fatty liver was significantly reduced and the serum aspartate transaminase (AST) and alanine transaminase (ALT) levels were much lower. Ethanol treatment significantly elevated hepatic CYP2E1 expression while simultaneously reducing hepatic phospho-acetyl CoA carboxylase (p-ACC) and carnitine palmitoyltransferase 1 (CPT-1) levels. While EGCG markedly reversed the effect of ethanol on hepatic p-ACC and CPT-1 levels, it had no effect on the ethanol-induced elevation in CYP2E1 expression. EGCG prevents ethanol-induced hepatotoxicity and inhibits the development of a fatty liver. These effects were associated with improvements in p-ACC and CPT-1 levels. The use of EGCG might be useful in treating patients with an alcoholic fatty liver.