In vitro covalent binding proteins of zerumbone, a chemopreventive food factor

Zerumbone (ZER), a sesquiterpene in Zingiber zerumbet Smith, has been implicated as a promising chemopreventive agent. Here we found that ZER suppressed expression of pro-inflammatory genes (COX-2 and iNOS) and induced detoxification genes (GSTP1 and NQO1) in RAW264.7 macrophages. Using the ZER-boun...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-08, Vol.73 (8), p.1905-1907
Main Authors: Ohnishi, K.(Kyoto Univ. (Japan)), Irie, K, Murakami, A
Format: Article
Language:English
Subjects:
affinity chromatography
Animals
anti-inflammation
ANTI-INFLAMMATOIRE
Anti-Inflammatory Agents - chemistry
Anti-Inflammatory Agents - metabolism
Anti-Inflammatory Agents - pharmacology
ANTIINFLAMMATORY AGENTS
ANTINFLAMATORIOS
Biological and medical sciences
Cell Line
Chemoprevention
detoxification
EXPRESION GENICA
EXPRESSION DES GENES
Food
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
Gene Expression Regulation - drug effects
GENES
Humans
MACROFAGOS
MACROPHAGE
MACROPHAGES
Macrophages - drug effects
Macrophages - metabolism
MICE
Protein Binding
Proteins - analysis
Proteins - chemistry
Proteins - metabolism
RATON
Rats
RAW264.7
RHIZOME
RHIZOMES
RIZOMAS
Sesquiterpenes - chemistry
Sesquiterpenes - metabolism
Sesquiterpenes - pharmacology
SESQUITERPENOIDE
SESQUITERPENOIDS
SESQUITERPENOS
SOURIS
Sulfhydryl Compounds - chemistry
zerumbone
ZINGIBER
Zingiber zerumbet
Zingiberaceae - chemistry
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Summary:Zerumbone (ZER), a sesquiterpene in Zingiber zerumbet Smith, has been implicated as a promising chemopreventive agent. Here we found that ZER suppressed expression of pro-inflammatory genes (COX-2 and iNOS) and induced detoxification genes (GSTP1 and NQO1) in RAW264.7 macrophages. Using the ZER-bound Sepharose gel, it appeared ZER was covalently bound to proteins, Keap1 and HuR, that play key roles in these molecular mechanisms.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.90265